Human ICAM-3 / CD50 (His Tag) recombinant protein

Cat.#: 503178

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Product Information

  • Product Name
    Human ICAM-3 / CD50 (His Tag) recombinant protein
  • Documents
  • Description
    The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
  • Protein name
    HCG2033729, isoform CRA_a
  • Protein short names
    CDW50; ICAM-R; ICAM-3; CD50; ICAM3
  • Uniprot ID
    P32942
  • Gene Name
    hCG_2033729
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human ICAM3 consists of 467 amino acids after removal of the signal peptide and predicts a molecular mass of 50.8 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3 is approximately 100-120 kDa due to the glycosylation.
  • Purity
    > 92 % as determined by SDS-PAGE
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
    When cells are added to ICAM3 coated plates (12.5 μg/mL , 100 μL/well), approximately >25% cells will adhere specifically.
  • Validations

    Human CD50 / ICAM-3 Protein (His Tag) SDS-PAGE

    Human CD50 / ICAM-3 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"