Human ICAM-3 / CD50 (His & Fc Tag) recombinant protein

Human ICAM-3 / CD50 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

HCG2033729, isoform CRA_a

CDW50; ICAM-R; ICAM-3; CD50; ICAM3

P32942

hCG_2033729

Human Cells

The extracellular domain (Met 1-His 485) of human ICAM3 (NP_002153.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human ICAM3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 703 amino acids and predicts a molecular mass of 77.2 kDa. By SDS-PAGE, the apparent molecular mass of rh ICAM3/Fc is approximately 125-135 kDa due to the glycosylation.

> 97 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3 coated plates (12.5 μg/ml, 100 μl/well), approximately 45%-60% will adhere specifically.