-
Product Name
Human HIF-1 alpha/HIF1A (His Tag) recombinant protein
- Documents
-
Description
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
-
Protein name
Hypoxia-inducible factor 1-alpha
-
Protein short names
PASD8; HIF-1ALPHA; HIF-1A; HIF1-ALPHA; HIF1A; BHLHE78; HIF1; MOP1; AA959795; HIF1ALPHA; HIF-1 ALPHA
-
Uniprot ID
Q16665
-
Gene Name
HIF1A; BHLHE78; MOP1; PASD8
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
A DNA sequence encoding the human HIF1A isoform 1 (Q16665-1) N-terminal segment (Arg 575-Asn 826) was expressed, with a polyhistide tag at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human HIF1A consisting of 259 amino acids and has a calculated molecular mass of 28.4 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
-
Purity
> 95 % as determined by SDS-PAGE
-
Validations
Human HIF-1 alpha / HIF1A Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"