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Product Name
Anti-HIF-1 alpha Rabbit antibody
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Description
HIF-1 alpha Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human HIF-1 alpha
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/30
FC: 1/30
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Validations
Western blot analysis of HIF-1 alpha expression in Ramos cell lysate.
Immunohistochemical analysis of paraffin-embedded human kindey, using HIF-1 alpha Antibody.
Immunofluorescent analysis of Hela cells, using HIF-1 alpha Antibody.
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Background
Swiss-Prot Acc.Q16665.Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"