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Product Name
Human Thrombopoietin/THPO (His Tag) recombinant protein
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Description
Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.
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Protein name
Thrombopoietin
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Protein short names
THPO; TPO-1; MGC163194; THCYT1; THROMBOPOIETIN; RP23-148P12.1; TPO-4; MKCSF; TPO; MPLLG; TPO-2; ML; MGDF; TPO-3
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Uniprot ID
P40225
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Gene Name
THPO; MGDF
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of human THPO (NP_000451.1) (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 3-15 ng/ml.
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Validations
Human Thrombopoietin / THPO / TPO Protein (His Tag) SDS-PAGE
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