Human MKK6/MEK6/MAP2K6 (207 Ser/Asp, 211 Thr/Asp, His & GST Tag) recombinant protein

Cat.#: 503703

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Product Information

  • Product Name
    Human MKK6/MEK6/MAP2K6 (207 Ser/Asp, 211 Thr/Asp, His & GST Tag) recombinant protein
  • Documents
  • Description
    Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases. {PubMed:10961885, PubMed:11727828, PubMed:15550393, PubMed:20869211, PubMed:8622669, PubMed:8626699, PubMed:8663074, PubMed:9218798}.
  • Protein name
    Dual specificity mitogen-activated protein kinase kinase 6
  • Protein short names
    MEK6; MKK6; PRKMK6; SKK3
  • Uniprot ID
    P52564
  • Gene Name
    MAP2K6; MEK6; MKK6; PRKMK6; SKK3
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human MAP2K6(S207D&T211D?/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 88 % as determined by SDS-PAGE
  • Activity
    The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.
  • Validations

    Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag) SDS-PAGE

    Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag) SDS-PAGE

    Human Vitronectin / VTN Protein (His Tag) SDS-PAGE

    Human Vitronectin / VTN Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"