Human DPP4 / CD26 (Fc Tag) recombinant protein

Human DPP4 / CD26 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Dipeptidyl peptidase 4

DPP4; THAM; ADABP; CD26; TP103; DPPIV; ADCP2; DPP-4

P27487

DPP4; ADCP2; CD26

Human Cells

A DNA sequence encoding the extracellular domain (Asn 29-Pro 766) of the mature form of human DPPIV (NP_001926.2) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Human

The recombinant human Fc/DPPIV is a disulfide-linked homodimeric protein.The reduced monomer consists of 975 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/DPPIV monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA.
2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA.
3. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV (Cat: 504194) bound to Spike (HCoV-EMC/2012) (Cat: 503810) was 11 nM.
4. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV (Cat: 504194) bound to Spike (HCoV-EMC/2012) (Cat: 503542) was 32 nM.
5. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV (Cat: 504194) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 503176) was 43 nM.
6. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV (Cat: 504194) bound to Spike-His (aa 1-760) (Cat: 500181) was 12 nM.