Human DPP4 / CD26 recombinant protein

Human DPP4 / CD26 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

Dipeptidyl peptidase 4

ADABP; DPP4; THAM; ADCP2; DPP-4; DPPIV; TP103; CD26

P27487

DPP4; ADCP2; CD26

Human Cells

The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.

Human

The recombinant human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.

> 70 % as determined by SDS-PAGE

1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg.
2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 502818) bound to Spike (HCoV-EMC/2012) (Cat: 503810) was 20 nM.
3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 502818) bound to Spike (HCoV-EMC/2012) (Cat: 503542) was 50 nM.
4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 502818) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 503176) was 33 nM.
5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 502818) bound to Spike-His (aa 1-760) (Cat: 500181) was 12 nM.