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Product Name
HSPA1L antibody
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Description
HSPA1L Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells, MCF7 cells, rat testis tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human endometrial cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Heat shOCk 70 kDa protein 1L antibody; HSP70 1L antibody; HSP70 HOM antibody; HSP70T antibody; HSPA1L antibody; hum70t antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSPA1L recombinant protein (Accession Number: NM_005527). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:111573(HSPA1L antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:111573(HSPA1L antibody) at dilution of 1:100 (under 40x lens)
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111573(HSPA1L Antibody) at dilution of 1:1000
IP Result of anti-HSPA1L (IP:Catalog No:111573, 4ug; Detection:Catalog No:111573 1:1000) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:111573(HSPA1L Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"