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HSP70 antibody

Cat.#: 111568

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Product Information

  • Product Name
    HSP70 antibody
  • Documents
  • Description
    HSP70 Rabbit Polyclonal antibody. Positive WB detected in multi-cells/tissue, A431 cells, A549 cells, COLO 320 cells, HepG2 cells, human brain tissue, Jurkat cells, MCF7 cells, mouse uterus tissue, PC-3 cells. Positive IP detected in mouse brain tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 66-70 kDa
  • Tested applications
    ELISA, IHC, WB, IF, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ54303 antibody; FLJ54370 antibody; FLJ54392 antibody; FLJ54408 antibody; FLJ75127 antibody; Heat shOCk 70 kDa protein 1/2 antibody; heat shOCk 70kDa protein 1A antibody; HSP70 antibody; HSP70 1 antibody; HSP70 1/HSP70 2 antibody; HSP70 1A antibody; HSP70.1/HSP70.2 antibody; HSP70I antibody; HSP72 antibody; HSPA1 antibody; HSPA1A antibody; HSPA1B antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HSP70 recombinant protein (Accession Number: NM_005346). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    WB result of Catalog No:111568(HSPA1A antibody).

    WB result of Catalog No:111568(HSPA1A antibody).

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111568(HSP70 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111568(HSP70 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody Catalog No:111568 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody Catalog No:111568 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-HSP70 (IP:Catalog No:111568, 3ug; Detection:Catalog No:111568 1:1000) with mouse brain tissue lysate 3600ug.

    IP Result of anti-HSP70 (IP:Catalog No:111568, 3ug; Detection:Catalog No:111568 1:1000) with mouse brain tissue lysate 3600ug.

    1X10^6 HepG2 cells were stained with 0.2ug HSP70 antibody (Catalog No:111568, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug HSP70 antibody (Catalog No:111568, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. This antibody well recognized the endogenous HSPA1A protein in multiple cell lines. (21373891)
  • References
    • Yang L, Wang Y, Zhang Q. Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse Sertoli cells. Molecular reproduction and development. 81(6):514-23. 2014.
    • Peng L, Liu M, Chang X. Role of the nucleus tractus solitarii in the protection of pre-moxibustion on gastric mucosal lesions. Neural regeneration research. 9(2):198-204. 2014.
    • Peng L, Liu M, Chang X. Effects of pre-moxibustion at Zusanli (ST36) on heat shock protein 70 expression in rats with gastric mucosal lesions after neurotomy. Neural regeneration research. 7(30):2370-6. 2012.
    • Gritsyna YV, Abdusalamova ZR, Vikhlyantsev IM. Changes in gene expression and content of Hsp70 and Hsp90 in striated muscles of mice after 30-day space flight on the biosatellite Bion-M1. Doklady. Biochemistry and biophysics. 463(1):199-202. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"