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Product Name
HSP47 antibody
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Description
HSP47 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human hepat℃irrhosis tissue. Positive FC detected in HepG2 cells. Positive WB detected in HT-1080 cells, A2780 cells, A431 cells, HeLa cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 46-48 kDa
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Tested applications
ELISA, IHC, WB, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
47 kDa heat shOCk protein antibody; AsTP3 antibody; CBP1 antibody; CBP2 antibody; Collagen binding protein antibody; Colligin antibody; gp46 antibody; HSP47 antibody; PIG14 antibody; PPROM antibody; RA A47 antibody; Serpin H1 antibody; SERPINH1 antibody; SERPINH2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSP47 recombinant protein (Accession Number: XM_024448756). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:111566(SERPINH1 antibody) at dilution of 1:100
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:111566(SERPINH1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:111566(SERPINH1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug SERPINH1 antibody (Catalog No:111566, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
SERPINH1 represents serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"