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Product Name
HSF2 Polyclonal Antibody
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Description
Polyclonal antibody to HSF2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
HSF2 antibody; HSF 2 antibody; HSTF 2 antibody; HSTF2 antibody; heat shock factor protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human HSF2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - HSF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HSF2 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - HSF2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using HSF2 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - HSF2 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using HSF2 antibody . Blue: DAPI for nuclear staining.
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Background
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"