-
Product Name
HSF2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to HSF2
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
HSF2 antibody; HSF 2 antibody; HSTF 2 antibody; HSTF2 antibody; heat shock factor protein 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 377-536 of human HSF2 (NP_004497.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - HSF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HSF2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
-
Background
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"