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Product Name
HSF1 antibody
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Description
HSF1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human cervical cancer tissue. Positive WB detected in K-562 cells, HepG2 cells, MCF7 cells, mouse kidney tissue, mouse spleen tissue, mouse testis tissue. Observed molecular weight by Western-blot: 68-80kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Heat shOCk factor protein 1 antibody; HSF 1 antibody; HSF1 antibody; HSTF 1 antibody; HSTF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSF1 recombinant protein (Accession Number: NM_005526). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111557(HSF1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:111557(HSF1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:111557(HSF1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
HSF1 belongs to heat-shock transcription factor that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishs a role for SIRT1 in protein homeostasis and heat-shock response.
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References
- Cui J, Tian H, Chen G. Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer. The Annals of thoracic surgery. 100(2):465-72. 2015.
- Aziz F, Wang X, Liu J, Yan Q. Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions. Toxicology in vitro : an international journal published in association with BIBRA. 31:158-66. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"