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Product Name
HSD11B2 Polyclonal Antibody
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Description
Polyclonal antibody to HSD11B2
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
HSD11B2 antibody; AME antibody; AME1 antibody; HSD11K antibody; HSD2 antibody; SDR9C3 antibody; hydroxysteroid 11-beta dehydrogenase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - HSD11B2 Polyclonal Antibody
Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 15s.
Immunohistochemistry - HSD11B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using HSD11B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HSD11B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HSD11B2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"