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Product Name
HSD11B2 antibody
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Description
HSD11B2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive WB detected in human kidney tissue, human placenta tissue, mouse kidney tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 40-43 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
11 beta HSD2 antibody; 11 DH2 antibody; AME antibody; AME1 antibody; HSD11B2 antibody; HSD11K antibody; HSD2 antibody; SDR9C3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HSD11B2 recombinant protein (Accession Number: NM_000196). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:111543(HSD11B2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111543(HSD11B2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111543(HSD11B2 antibody) at dilution of 1:50 (under 40x lens)
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Background
The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).
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References
- Moravec M, Svec J, Ergang P. Expression of 11β-hydroxysteroid dehydrogenase type 2 is deregulated in colon carcinoma. Histology and histopathology. 29(4):489-96. 2014.
- Tetsi Nomigni M, Ouzounian S, Benoit A. Steroidogenic enzyme profile in an androgen-secreting adrenocortical oncocytoma associated with hirsustism. Endocrine connections. 4(2):117-27. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"