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Product Name
HRAS-Specific antibody
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Description
HRAS-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, NIH/3T3 cells, PC-3 cells. Positive FC detected in HeLa cells. Positive IHC detected in human kidney tissue, human prostate hyperplasia tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, IF, IHC, WB, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C BAS/HAS antibody; C H RAS antibody; C HA RAS1 antibody; CTLO antibody; GTPase HRas antibody; H Ras 1 antibody; H RASIDX antibody; Ha Ras antibody; HAMSV antibody; HRAS antibody; HRAS1 antibody; K RAS antibody; p21ras antibody; RASH1 antibody; Transforming protein p21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_176795). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:111460(HRAS-Specific Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:111460(HRAS-Specific Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:111460(HRAS-Specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111460(HRAS-Specific Antibody) at dilution of 1:300
1X10^6 HeLa cells were stained with 1ug HRAS-Specific antibody (Catalog No:111460, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
HRAS,also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can’t recognize NRAS and KRAS.
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References
- Cai WY, Wei TZ, Luo QC. The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. Journal of cell science. 126(Pt 13):2877-89. 2013.
- Ceccarelli V, Nocentini G, Billi M. Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. PloS one. 9(1):e85025. 2014.
- Liu W, Ning R, Chen RN. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells. Molecular carcinogenesis. 2015.
- Park SJ, Shim JW, Park HS. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"