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Product Name
HPSE antibody
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Description
HPSE Rabbit Polyclonal antibody. Positive IF detected in HeLa cells, HepG2 cells. Positive IHC detected in human liver cancer tissue, human placenta tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Endo glucoronidase antibody; HEP antibody; heparanase antibody; Heparanase 1 antibody; HPA antibody; Hpa1 antibody; HPR1 antibody; HPSE antibody; HPSE1 antibody; HSE1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HPSE recombinant protein (Accession Number: NM_001098540). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111456(HPSE Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer slide using Catalog No:111456(HPSE Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver cancer slide using Catalog No:111456(HPSE Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:111456(HPSE Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.
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References
- Wang X, Zuo D, Chen Y. Shed Syndecan-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer. British journal of cancer. 111(10):1965-76. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"