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Product Name
HPR Polyclonal Antibody
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Description
Polyclonal antibody to HPR
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
HPR antibody; A-259H10.2 antibody; HP antibody; haptoglobin-related protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-348 of human HPR (NP_066275.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - HPR Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HPR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - HPR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using HPR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HPR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using HPR antibody at dilution of 1:100 (40x lens).
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Background
Primate-specific plasma protein associated with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL). This HDL particle, termed trypanosome lytic factor-1 (TLF-1), mediates human innate immune protection against many species of African trypanosomes. Binds hemoglobin with high affinity and may contribute to the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"