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Product Name
HPDL antibody
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Description
HPDL Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive IHC detected in human stomach. Positive WB detected in L02 cells. Observed molecular weight by Western-blot: 35-39kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
human; other species not tested.
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Alternative names
HPDL antibody; GLOXD1 antibody; RP4 534D1.1 antibody; 4 HPPD L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HPDL recombinant protein (Accession Number: NM_032756). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:111447(HPDL antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human stomach using Catalog No:111447(HPDL antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:111447(HPDL antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using HPDL antibody Catalog No:111447 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
HPDL represents 4-hydroxyphenylpyruvate dioxygenase-like
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