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Product Name
HPCAL1 Polyclonal Antibody
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Description
Polyclonal antibody to HPCAL1
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HPCAL1 antibody; BDR1 antibody; HLP2 antibody; VILIP-3 antibody; hippocalcin like 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:20 - 1:100 -
Validations
Western blot - HPCAL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HPCAL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - HPCAL1 Polyclonal Antibody
Western blot analysis of extracts of various cells, using HPCAL1 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunoprecipitation - HPCAL1 Polyclonal Antibody
Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody . Western blot was performed from the immunoprecipitate using HPCAL1 antibody at a dilition of 1:1000.
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Background
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"