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Product Name
HOMER1 antibody
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Description
HOMER1 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in mouse brain tissue, human ovary tumor tissue, human pancreas tissue. Positive WB detected in human brain tissue, human/mouse brain. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 46-48 kDa
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Tested applications
ELISA, WB, FC, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HOMER antibody; Homer 1 antibody; homer homolog 1 (Drosophila) antibody; Homer protein homolog 1 antibody; HOMER1 antibody; HOMER1A antibody; HOMER1B antibody; HOMER1C antibody; SYN47 antibody; Ves 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HOMER1 recombinant protein (Accession Number: NM_004272). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of anti-HOMER1 (Catalog No:) with mouse brain lysate and human brain lysate.
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111517(HOMER1 antibody) at dilution of 1:1000
IP Result of anti-HOMER1 (IP:Catalog No:111517, 4ug; Detection:Catalog No:111517 1:800) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:111517(HOMER1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:111517(HOMER1 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug HOMER1 antibody (Catalog No:111517, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.
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References
- Su J, Tang Y, Zhou H, Liu L, Dong Q. Tissue kallikrein protects neurons from hypoxia/reoxygenation-induced cell injury through Homer1b/c. Cellular signalling. 24(11):2205-15. 2012.
- Wu YY, Wang X, Tan L. Lithium attenuates scopolamine-induced memory deficits with inhibition of GSK-3β and preservation of postsynaptic components. Journal of Alzheimer's disease : JAD. 37(3):515-27. 2013.
- Su JJ, Pan H, Zhou HG, Tang YP, Dong Q, Liu JR. Acid-sensing ion channels activation and hypoxia upregulate Homer1a expression. CNS neuroscience & therapeutics. 20(3):264-74. 2014.
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