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Product Name
HNRNPK Polyclonal Antibody
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Description
Polyclonal antibody to HNRNPK
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HNRNPK antibody; AUKS antibody; CSBP antibody; HNRPK antibody; TUNP antibody; heterogeneous nuclear ribonucleoprotein K antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPK (NP_112552.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - HNRNPK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HNRNPK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPK Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPK Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using HNRNPK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using HNRNPK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - HNRNPK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPK Antibody at dilution of 1:200 (40x lens).
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Background
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"