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Product Name
Anti-hnRNP K Rabbit antibody
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Description
hnRNP K Rabbit monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Alternative names
AUKS; CSBP; TUNP; HNRPK antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human hnRNP K
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
IHC: 1/20-1/50
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Validations
Western blot detection of hnRNP K in K562,PC3,Hela cell lysates using hnRNP K Rabbit mAb(1:500 diluted).Predicted band size:51kDa.Observed band size:62kDa.
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Background
Swiss-Prot Acc.P61978.One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction . As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
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