HNF4G Rabbit Polyclonal antibody. Positive IHC detected in human small intestine tissue. Positive FC detected in HEK-293 cells. Positive WB detected in mouse pancreas tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 46 kDa
ELISA, WB, IHC, FC
Human, Mouse; other species not tested.
HNF 4 gamma antibody; HNF4G antibody; NR2A2 antibody; NR2A3 antibody
This antibody was obtained by immunization of HNF4G recombinant protein (Accession Number: XM_017013376). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:111497(HNF4G Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111497(HNF4G Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111497(HNF4G Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HEK-293 cells were stained with 0.2ug HNF4G antibody (Catalog No:111497, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
HNF4 (hepatocyte nuclear factor 4) is a nuclear receptor protein that has a critical role in hepatocyte differentiation and function (PMID: 12774000). Two isoforms of human HNF4 exist, HNF4-alpha and HNF4-gamma, which are encoded by two separate genes HNF4A and HNF4G respectively (PMID: 7926813). HNF4-gamma has a lower transcription activation potential than HNF4-alpha. HNF4-gamma is expressed in kidney, gut, pancreas, and testis (PMID: 16945327). It has been reported to be involved in cancer progression (PMID: 23896584). This polyclonal antibody is raised against C-terminal 82 amino acids of human HNF4-gamma.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"