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Product Name
HNF4G antibody
- Documents
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Description
HNF4G Rabbit Polyclonal antibody. Positive IHC detected in human small intestine tissue. Positive FC detected in HEK-293 cells. Positive WB detected in mouse pancreas tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HNF 4 gamma antibody; HNF4G antibody; NR2A2 antibody; NR2A3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HNF4G recombinant protein (Accession Number: XM_017013376). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:111497(HNF4G Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111497(HNF4G Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:111497(HNF4G Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HEK-293 cells were stained with 0.2ug HNF4G antibody (Catalog No:111497, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
HNF4 (hepatocyte nuclear factor 4) is a nuclear receptor protein that has a critical role in hepatocyte differentiation and function (PMID: 12774000). Two isoforms of human HNF4 exist, HNF4-alpha and HNF4-gamma, which are encoded by two separate genes HNF4A and HNF4G respectively (PMID: 7926813). HNF4-gamma has a lower transcription activation potential than HNF4-alpha. HNF4-gamma is expressed in kidney, gut, pancreas, and testis (PMID: 16945327). It has been reported to be involved in cancer progression (PMID: 23896584). This polyclonal antibody is raised against C-terminal 82 amino acids of human HNF4-gamma.
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