HMMR-Specific antibody

Cat.#: 111490

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Product Information

  • Product Name
    HMMR-Specific antibody
  • Documents
  • Description
    HMMR-Specific Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 70 kDa
  • Tested applications
    ELISA, WB, IF, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CD168 antibody; HMMR antibody; IHABP antibody; RHAMM antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_001142556). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111490(HMMR-Specific antibody) at dilution of 1:800

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111490(HMMR-Specific antibody) at dilution of 1:800

    Immunofluorescent analysis of HepG2 cells, using HMMR antibody Catalog No:111490 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using HMMR antibody Catalog No:111490 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HepG2 cells were stained with 0.2ug HMMR-Specific antibody (Catalog No:111490, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with 0.2ug HMMR-Specific antibody (Catalog No:111490, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    HMMR, also named RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus playing roles in wound healing and tumor metastasis. HMMR was expressed as a 70 kDa protein in many tissues (PMID: 7590272). This antibody can recognize HMMR from Human, Mouse and Rat.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"