HMGB2 antibody

HMGB2 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

HMGB2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in Jurkat cells, HEK-293 cells, HL-60 cells, human cerebellum tissue, K-562 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 33-35 kDa

ELISA, WB, IHC, IP

Human,Mouse,Rat; other species not tested.

high mobility group box 2 antibody; High mobility group protein 2 antibody; High mobility group protein B2 antibody; HMG 2 antibody; HMG2 antibody; HMGB2 antibody

Rabbit IgG

This antibody was obtained by immunization of HMGB2 recombinant protein (Accession Number: NM_001130688). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

High mobility group protein B2 (HMGB2) belongs to a family of highly conserved proteins that contain HMG box domains (11246022,14871457). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (14871457). HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals (11262228). HMGB3 is only expressed during embryogenesis (9598312). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix . HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 and HMGB2 facilitate the binding of Hox proteins, oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (11246022,14871457). Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes (19317908 ,10490593). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation endproducts (RAGE) (19811285 ). Research studies have shown that HMGB2 overexpression in hepatocellular carcinoma is associated with poor prognosis and shorter survival time (20851854).This antibody recognizes the phosphorylation form of HMGB2 protein.

• Abraham AB, Bronstein R, Chen EI. Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells. Proteome science. 11(1):18. 2013.