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Product Name
HMG20A antibody
- Documents
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Description
HMG20A Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10739 antibody; high mobility group 20A antibody; HMG box containing protein 20A antibody; HMG20A antibody; HMGX1 antibody; HMGXB1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HMG20A recombinant protein (Accession Number: XM_047432108). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111475(HMG20A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111475(HMG20A antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using HMG20A antibody Catalog No:111475 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HMG20A (IP:Catalog No:111475, 4ug; Detection:Catalog No:111475 1:500) with Jurkat cells lysate 3200ug.
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Background
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation
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References
- Davies RG, Wagstaff KM, McLaughlin EA, Loveland KL, Jans DA. The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins. Biochimica et biophysica acta. 1833(12):3436-44. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"