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HLA-F antibody

Cat.#: 111430

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Product Information

  • Product Name
    HLA-F antibody
  • Documents
  • Description
    HLA-F Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue, human placenta tissue, human spleen tissue. Positive WB detected in human placenta tissue, A431 cells, human spleen tissue, Raji cells. Observed molecular weight by Western-blot: 45 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CDA12 antibody; HLA 5.4 antibody; HLA CDA12 antibody; HLA F antibody; HLA F antigen antibody; HLAF antibody; HLA-F antibody; LeukOCyte antigen F antibody; MHC class I antigen F antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HLA-F recombinant protein (Accession Number: NM_018950). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111430(HLA-F antibody) at dilution of 1:500

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111430(HLA-F antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:111430(HLA-F antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:111430(HLA-F antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:111430(HLA-F antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:111430(HLA-F antibody) at dilution of 1:100 (under 40x lens)

  • Background
    Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)
  • References
    • Ishigami S, Arigami T, Setoyama T. Clinical-pathological implication of human leukocyte antigen-F-positive gastric adenocarcinoma. The Journal of surgical research. 184(2):802-6. 2013.
    • Zhang X, Lin A, Zhang JG. Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma. International journal of cancer. 132(1):82-9. 2013.
    • Zhang JG, Zhang X, Lin A, Yan WH. Lesion HLA-F expression is irrelevant to prognosis for patients with gastric cancer. Human immunology. 74(7):828-32. 2013.
    • Lin A, Zhang X, Ruan YY, Wang Q, Zhou WJ, Yan WH. HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer. Lung cancer (Amsterdam, Netherlands). 74(3):504-9. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"