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HLA class I (HLA-B) antibody

Cat.#: 111415

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Product Information

  • Product Name
    HLA class I (HLA-B) antibody
  • Documents
  • Description
    HLA class I (HLA-B) Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue. Positive IP detected in HepG2 cells. Positive WB detected in Jurkat cells, HeLa cells, HepG2 cells, MCF7 cells. Observed molecular weight by Western-blot: 40-45kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    HLA B antibody; HLA B 7301 antibody; HLA B73 antibody; HLAB antibody; HLA-B antibody; HLAC antibody; MHC class I antigen B*7 antibody; SPDA1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of HLA class I (HLA-B) recombinant protein (Accession Number: BC013187). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:800

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human spleen using Catalog No:111415(HLA class I (HLA-B) antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-HLA class I (HLA-B) (IP:Catalog No:111415, 4ug; Detection:Catalog No:111415 1:2000) with HepG2 cells lysate 4000ug.

    IP Result of anti-HLA class I (HLA-B) (IP:Catalog No:111415, 4ug; Detection:Catalog No:111415 1:2000) with HepG2 cells lysate 4000ug.

    Immunofluorescent analysis of HepG2 cells using Catalog No:111415(HLA class I (HLA-B) Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:111415(HLA class I (HLA-B) Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"