Histone H4 Polyclonal Antibody

Cat.#: 160721

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Product Information

  • Product Name
    Histone H4 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to Histone H4
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HIST4H4 antibody; H4/p antibody; histone H4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human Histone H4 (NP_001029249.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
  • Validations

    Western blot - Histone H4 Polyclonal Antibody

    Western blot - Histone H4 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using Histone H4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - Histone H4 Polyclonal Antibody

    Immunohistochemistry - Histone H4 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using Histone H4 antibody at dilution of 1:200 (40x lens).

  • Background
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"