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Product Name
Histone H2A.X antibody
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Description
Histone H2A.X Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Positive WB detected in Raji cells, HEK-293 cells, HL-60 cells, mouse heart tissue. Observed molecular weight by Western-blot: 15-18kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
H2A histone family antibody; member X antibody; H2A.X antibody; H2A/X antibody; H2AFX antibody; H2AX antibody; Histone H2A.x antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Histone H2A.X recombinant protein (Accession Number: NM_002105). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:111404(H2AFX antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111404(H2AFX antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using H2AFX antibody Catalog No:111404 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug Histone H2A.X antibody (Catalog No:111404, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
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References
- Dickey JS, Redon CE, Nakamura AJ, Baird BJ, Sedelnikova OA, Bonner WM. H2AX: functional roles and potential applications. Chromosoma. 118(6):683-92. 2009.
- Wu Y, Sun L, Zou W. Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth. Cancer science. 103(10):1820-5. 2012.
- Chen X, Zhang Z, Feng Y. Aberrant survivin expression in endometrial hyperplasia: another mechanism of progestin resistance. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 22(5):699-708. 2009.
- Wang Y, Huang Y, Guan F. Hypoxia-inducible factor-1alpha and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation. PloS one. 8(9):e74051. 2013.
- Qi W, Wang R, Chen H. BRG1 promotes the repair of DNA double-strand breaks by facilitating the replacement of RPA with RAD51. Journal of cell science. 128(2):317-30. 2015.
- Yaglom JA, McFarland C, Mirny L, Sherman MY. Oncogene-triggered suppression of DNA repair leads to DNA instability in cancer. Oncotarget. 5(18):8367-78. 2014.
- Wu P, Zhu X, Jin W, Hao S, Liu Q, Zhang L. Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells. Biochemical and biophysical research communications. 460(2):183-90. 2015.
- Xu Y, Wu D, Fan Y. Novel Recombinant Protein FlaA N/C Protects against Radiation Injury via NF-κB Signaling. Radiation research. 185(1):77-86. 2016.
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