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Product Name
Anti-Phospho-Histone H3 (Ser139) Rabbit antibody
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Description
Phospho-Histone H3 (Ser139) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/200
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Validations
Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139) Antibody .
Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Histone H2A.X (Phospho-Ser139) Rabbit pAb (166716,1:500 diluted,upper) or Beta actin Mouse mAb (200068-8F10,lower).
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Background
Swiss-Prot Acc.P16104.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"