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Product Name
HINFP antibody
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Description
HINFP Rabbit Polyclonal antibody. Positive IF detected in Hela cells, L02 cells. Positive WB detected in human liver tissue, L02 cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434F162 antibody; HiNF P antibody; HINFP antibody; Histone nuclear factor P antibody; MIZF antibody; ZNF743 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HINFP recombinant protein (Accession Number: NM_015517). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111350(HINFP antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using HINFP antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"