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Product Name
Hikeshi antibody
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Description
Hikeshi Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, A375 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human lung tissue. Positive IF detected in A375 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C11orf73 antibody; FLJ43020 antibody; Hikeshi antibody; HSPC138 antibody; HSPC179 antibody; HSPC248 antibody; L7RN6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Hikeshi recombinant protein (Accession Number: NM_016401). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111348(C11orf73 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung using Catalog No:111348(C11orf73 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:111348(C11orf73 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-C11orf73 (IP:Catalog No:111348, 4ug; Detection:Catalog No:111348 1:500) with mouse brain tissue lysate 3000ug.
Immunofluorescent analysis of A375 cells using Catalog No:111348(C11orf73 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
C11orf73 represents chromosome 11 open reading frame 73
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"