-
Product Name
HGD antibody
- Documents
-
Description
HGD Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in human liver tissue, HeLa cells. Observed molecular weight by Western-blot: 50kd
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
AKU antibody; FLJ94126 antibody; HGD antibody; HGO antibody; Homogentisate 1 antibody; 2 dioxygenase antibody; Homogentisate oxygenase antibody; Homogentisic acid oxidase antibody; Homogentisicase antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of HGD recombinant protein (Accession Number: BC020792). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111313(HGD antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:111313(HGD antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111313(HGD antibody) at dilution of 1:100 (under 40x lens)
-
Background
Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"