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Product Name
Hexokinase 2 antibody
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Description
Hexokinase 2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, A431 cells, HeLa cells, K-562 cells, MCF7 cells. Positive IHC detected in human skeletal muscle tissue. Observed molecular weight by Western-blot: 102kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686M1669 antibody; Hexokinase 2 antibody; Hexokinase type II antibody; HK II antibody; HK2 antibody; HKII antibody; HXK2 antibody; Muscle form hexokinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Hexokinase 2 recombinant protein (Accession Number: NM_000189). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111307(HK2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:111307(HK2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:111307(HK2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-HK2 (IP:Catalog No:111307, 3ug; Detection:Catalog No:111307 1:1000) with HeLa cells lysate 2000ug.
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Background
HK2 represents hexokinase 2
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References
- Yan S, Zhang Q, Zhong X. I prostanoid receptor-mediated inflammatory pathway promotes hepatic gluconeogenesis through activation of PKA and inhibition of AKT. Diabetes. 63(9):2911-23. 2014.
- Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
- Zhuo B, Li Y, Li Z. PI3K/Akt signaling mediated Hexokinase-2 expression inhibits cell apoptosis and promotes tumor growth in pediatric osteosarcoma. Biochemical and biophysical research communications. 464(2):401-6. 2015.
- Zhou P, Chen WG, Li XW. MicroRNA-143 acts as a tumor suppressor by targeting hexokinase 2 in human prostate cancer. American journal of cancer research. 5(6):2056-63. 2015.
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