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Hemoglobin epsilon-Specific antibody

Cat.#: 107259

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Special Price 239.0 USD

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Product Information

  • Product Name
    Hemoglobin epsilon-Specific antibody
  • Documents
  • Description
    Hemoglobin epsilon-Specific Mouse Monoclonal antibody. Positive FC detected in K-562 cells. Positive WB detected in K-562 cells. Observed molecular weight by Western-blot: 16 kDa
  • Tested applications
    ELISA, WB, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Epsilon globin antibody; HBE antibody; Hemoglobin epsilon antibody; Hemoglobin epsilon chain antibody; Hemoglobin subunit epsilon antibody; hemoglobin antibody; epsilon 1 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of recombinant protein (Accession Number: NM_005330). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107259(HBE1-Specific antibody) at dilution of 1:500

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107259(HBE1-Specific antibody) at dilution of 1:500

    1X10^6 K-562 cells were stained with 0.2ug HBE1-Specific antibody (Catalog No:107259, red) and control antibody (blue). Fixed with blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

    1X10^6 K-562 cells were stained with 0.2ug HBE1-Specific antibody (Catalog No:107259, red) and control antibody (blue). Fixed with blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

  • Background
    The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"