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Product Name
HELB antibody
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Description
HELB Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in HEK-293 cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 120-140 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
DNA helicase B antibody; hDHB antibody; HELB antibody; helicase (DNA) B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HELB recombinant protein (Accession Number: NM_033647). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111399(HELB Antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:111399(HELB Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-HELB (IP:Catalog No:111399, 4ug; Detection:Catalog No:111399 1:1000) with HEK-293 cells lysate 3000ug.
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Background
HELB represents helicase (DNA) B
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