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Product Name
HECTD1 antibody
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Description
HECTD1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human kidney tissue, human brain tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue. Observed molecular weight by Western-blot: 290kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
E3 ligase for inhibin receptor antibody; EULIR antibody; FLJ38315 antibody; HECT domain containing 1 antibody; HECTD1 antibody; KIAA1131 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HECTD1 recombinant protein (Accession Number: BC006237). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111396(HECTD1 antibody) at dilution of 1:500
IP Result of anti-HECTD1 (IP:Catalog No:111396, 5ug; Detection:Catalog No:111396 1:500) with mouse brain tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111396(HECTD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111396(HECTD1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using HECTD1 antibody Catalog No:111396 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson’s disease(PMID:16714300).This protein is specific to HECTD1.
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References
- Li W, Hu Y, Oh S. Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation. Molecular cell. 59(2):188-202. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"