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Product Name
HDAC5 Polyclonal Antibody
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Description
Polyclonal antibody to HDAC5
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
HDAC5 antibody; HD5 antibody; NY-CO-9 antibody; histone deacetylase 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HDAC5 (NP_005465.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - HDAC5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HDAC5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary gland using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using HDAC5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HDAC5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC5 antibody at dilution of 1:100 (40x lens).
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Background
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"