-
Product Name
HCCS antibody
- Documents
-
Description
HCCS Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in human heart tissue, HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 31 kDa
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CCHL antibody; CytOChrome c type heme lyase antibody; DKFZp779I1858 antibody; HCCS antibody; HolOCytOChrome c type synthase antibody; MCOPS7 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of HCCS recombinant protein (Accession Number: NM_005333). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
-
Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111278(HCCS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111278(HCCS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111278(HCCS antibody) at dilution of 1:100 (under 40x lens)
-
Background
HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"