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Product Name
HBB antibody
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Description
HBB Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human placenta tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 13-16 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
beta globin antibody; CD113t C antibody; HBB antibody; Hemoglobin beta chain antibody; Hemoglobin subunit beta antibody; hemoglobin antibody; beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HBB recombinant protein (Accession Number: NM_000518). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111266(HBB antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human liver using Catalog No:111266(HBB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111266(HBB antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-HBB (IP:Catalog No:111266, 4ug; Detection:Catalog No:111266 1:1000) with mouse heart tissue lysate 3200ug.
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Background
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
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