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Product Name
HAX1 antibody
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Description
HAX1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ17042 antibody; FLJ18492 antibody; FLJ93803 antibody; HAX 1 antibody; HAX1 antibody; HCLS1 assOCiated protein X 1 antibody; HCLSBP1 antibody; HS1 assOCiating protein X 1 antibody; HS1 binding protein 1 antibody; HS1BP1 antibody; HSP1BP 1 antibody; SCN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HAX1 recombinant protein (Accession Number: NM_006118). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
IP Result of anti-HAX1 (IP:Catalog No:111263, 3ug; Detection:Catalog No:111263 1:300) with mouse brain tissue lysate 6000ug.
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111263(HAX1 antibody) at dilution of 1:50 (under 10x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111263(HAX1 Antibody) at dilution of 1:300
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Background
HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech’s anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.
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References
- Cavnar PJ, Berthier E, Beebe DJ, Huttenlocher A. Hax1 regulates neutrophil adhesion and motility through RhoA. The Journal of cell biology. 193(3):465-73. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"