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Product Name
Harmonin antibody
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Description
Harmonin Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 68-73 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AIE 75 antibody; AIE75 antibody; Antigen NY CO 38/NY CO 37 antibody; Harmonin antibody; NY CO 37 antibody; NY CO 38 antibody; PDZ 45 antibody; PDZ 73 antibody; PDZ 73/NY CO 38 antibody; PDZ73 antibody; USH1C antibody; Usher 1C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Harmonin recombinant protein (Accession Number: NM_001297764). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111255(USH1C antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111255(USH1C antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using USH1C antibody Catalog No:111255 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-USH1C (IP:Catalog No:111255, 4ug; Detection:Catalog No:111255 1:600) with HEK-293 cells lysate 3000ug.
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Background
USH1C represents Usher syndrome 1C (autosomal recessive, severe)
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References
- Jansen F, Kalbe B, Scholz P. Impact of the Usher syndrome on olfaction. Human molecular genetics. 25(3):524-33. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"