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Product Name
HADH Polyclonal Antibody
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Description
Polyclonal antibody to HADH
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
HADH antibody; HAD antibody; HADH1 antibody; HADHSC antibody; HCDH antibody; HHF4 antibody; MSCHAD antibody; SCHAD antibody; hydroxyacyl-CoA dehydrogenase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human HADH (NP_005318.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - HADH Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HADH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - HADH Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using HADH antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HADH Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using HADH antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HADH Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using HADH antibody at dilution of 1:100 (40x lens).
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Background
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"