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Product Name
GUF1 antibody
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Description
GUF1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, mouse pancreas tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human oesophagus tissue, human kidney tissue, human lung cancer tissue, human testis tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ13220 antibody; GUF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GUF1 recombinant protein (Accession Number: NM_021927). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111252(GUF1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human oesophagus using Catalog No:111252(GUF1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human oesophagus using Catalog No:111252(GUF1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GUF1 antibody Catalog No:111252 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GUF1 (IP:Catalog No:111252, 3ug; Detection:Catalog No:111252 1:500) with HeLa cells lysate 3200ug.
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Background
GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.
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