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Product Name
GUCY2F Polyclonal Antibody
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Description
Polyclonal antibody to GUCY2F
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Tested applications
WB, IF
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Species reactivity
Mouse, Rat
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Alternative names
GUCY2F antibody; CYGF antibody; GC-F antibody; GUC2DL antibody; GUC2F antibody; RETGC-2 antibody; ROS-GC2 antibody; retinal guanylyl cyclase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-460 of human GUCY2F (NP_001513.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - GUCY2F Polyclonal Antibody
Western blot analysis of extracts of mouse eye, using GUCY2F antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"