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Product Name
GTF2A2 antibody
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Description
GTF2A2 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, HeLa cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 12kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GTF2A2 antibody; HsT18745 antibody; TF2A2 antibody; TFIIA antibody; TFIIA 12 antibody; TFIIA gamma antibody; TFIIA p12 subunit antibody; TFIIAS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GTF2A2 recombinant protein (Accession Number: NM_001320929). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111228(GTF2A2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111228(GTF2A2 antibody) at dilution of 1:100 (under 25x lens)
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Background
Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region [PMID:20480367]. TFIIA in a complex with TBP mediates transcriptional activity [PMID: 11030333]. GTF2A2 is one subunit of TFIIA.
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