-
Product Name
GSTO1 antibody
- Documents
-
Description
GSTO1 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, human heart tissue, human liver tissue, mouse liver tissue, PC-3 cells, U-937 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 27-30kd
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DKFZp686H13163 antibody; GSTO 1 antibody; GSTO1 antibody; GSTTLp28 antibody; P28 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of GSTO1 recombinant protein (Accession Number: NM_004832). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111189(GSTO1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111189(GSTO1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111189(GSTO1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GSTO1 antibody Catalog No:111189 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GSTO1 (IP:Catalog No:111189, 4ug; Detection:Catalog No:111189 1:500) with Jurkat cells lysate 4000ug.
-
Background
GSTO1 represents glutathione S-transferase omega 1
-
References
- Chuang JJ, Dai YC, Lin YL. Downregulation of glutathione S-transferase M1 protein in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced mouse bladder carcinogenesis. Toxicology and applied pharmacology. 279(3):322-30. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"